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SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based)

🏷 GM-CD4-T-Cell-iso-kit
IndirectColumn-basedNon-releasable
Maximize your research potential with GeneMedi’s SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit. Specifically designed for Human CD4+ T Cell isolation from PBMC, this Column-based system employs a precise Untouched selection strategy.
SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) is a Indirect CD4+ T Cell Isolation Kit (Column-based, Non-releasable)
📄 MSDS

Informations de commande

N° de catalogue Nom du produit Taille Prix (EUR)
GM-CD4-T-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) 10 Tests EUR 176.70
GM-CD4-T-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) 100 Tests EUR 933.72
GM-CD4-T-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) 750 Tests
SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based)

Spécifications

  • Population cellulaire cible CD4-T Cell
  • Type de marquage Indirect
  • Type de bille Non-releasable
  • Format Column-based
  • Méthode d'isolement Negative CD4-T Cell Isolation (Untouched)
  • Conditions de stockage Conserver entre 2℃ et 8℃

Description

N° de catalogue GM-CD4-T-Cell-iso-kit
Nom du produit SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based)
Nom anglais SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based)
Description SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) is a Indirect CD4+ T Cell Isolation Kit (Column-based, Non-releasable)
Détails d'application SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) is used for Negative CD4+ T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Composants
  • GM-CD4-T-Cell-iso-Cocktail-1: SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Cocktail
  • GMP-SMT-219-Ab01-nanoIMB: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)
Type de marquage Indirect
Type de bille Non-releasable
Format Column-based
Caractéristique clé Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Population cellulaire cible CD4-T Cell
Conditions de stockage Conserver entre 2℃ et 8℃

Détails d'application

SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) is used for Negative CD4+ T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies

Protocole

  1. Sample Prep: Isolate PBMCs and resuspend in separation buffer.
  2. Labeling: Add the isolation cocktail to the sample and incubate for 5 minutes at room temperature.
  3. Bead Incubation: Add Anti-biotin Nanobeads and incubate for 10 minutes at 2-8°C.
  4. Separation: Apply the cell suspension to an LS Column placed in a magnetic field. Collect the flow-through, which contains the enriched, untouched CD4+ T cells.

Données de validation

Enrichissement efficace des cellules T CD4+ de haute pureté à partir de PBMC humains en utilisant SOLIDEX®-ISOEx Untouched Kit d'isolement des cellules T CD4+ humaines (basé sur colonne)

Numéro de catalogue GM-CD4-T-Cell-iso-kit
Pour évaluer l'efficacité d'isolement du SOLIDEX®-ISOEx Untouched Kit d'isolement des cellules T CD4+ humaines (basé sur colonne), les cellules T CD4+ ont été enrichies à partir de cellules mononucléées du sang périphérique humain (hPBMCs) par sélection négative. Les cellules non cibles ont été marquées avec un cocktail d'anticorps biotinylés et déplétées à l'aide de billes magnétiques dans une colonne de séparation.
Les fractions cellulaires ont été colorées avec CD3-APC et CD4-PE pour une analyse par cytométrie en flux. La fréquence des cellules T CD4+ a montré une augmentation substantielle de 23,3 % dans l'échantillon avant isolement à 94,4 % dans la fraction après isolement. Ces résultats confirment que le système basé sur colonne fournit une déplétion supérieure des populations non cibles, produisant des cellules T CD4+ "non touchées" hautement purifiées.

Figure 16. Isolement des cellules T CD4+ humaines à l'aide de SOLIDEX®-ISOEx Untouched Kit d'isolement des cellules T CD4+ humaines (basé sur colonne).Graphiques de cytométrie en flux représentatifs montrant la fréquence des cellules T CD4+ dans les hPBMCs avant isolement (23,3 %) et après enrichissement par sélection négative sur colonne (94,4 %).

ISOEx kit d'isolement cellulaire & Nanobeads validation
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Contexte

Why Choose SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit?

  • Zero Activation: The "Untouched" negative selection ensures CD4 receptors are not engaged by antibodies, preventing artificial signaling.
  • Superior Purity: The column-based matrix enhances separation efficiency, typically achieving higher purity compared to column-free methods.
  • Rapid Workflow: Complete the labeling and separation process in approximately 20 minutes.
  • Downstream Ready: Cells are immediately ready for flow cytometry staining or culture without bead interference.

FAQ

What are the sample requirements?
This kit is optimized for use with Peripheral Blood Mononuclear Cells (PBMC). Fresh PBMCs yield the highest viability, but the kit is also compatible with cryopreserved PBMCs.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Because this is an "Untouched" (negative) selection kit, the target CD4+ T cells are free of bound antibodies or magnetic beads, leaving all surface markers available for staining.
How does the column-based negative selection work?
Non-CD4+ cells (CD8+ T cells, B cells, NK cells, Monocytes, etc.) are magnetically labeled. When passed through the column in a magnetic field, these labeled cells are retained, while the unlabeled CD4+ T cells pass through freely.
Can the columns be reused?
No. The columns are single-use consumables designed to ensure sterility and prevent cross-contamination between samples.
What is the advantage of this column-based method over column-free methods?
Column-based separation utilizes a matrix that amplifies the magnetic field, allowing for the retention of cells with very weak magnetic labeling. This often results in higher purity and recovery compared to simple tube-based (column-free) magnets.
Is this workflow compatible with automation?
Yes, the magnetic labeling and separation steps can be adapted for use with automated liquid handling systems and magnetic bead processors.

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