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SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based)

🏷 GM-CD8-T-Cell-iso-kit
IndirectColumn-basedNon-releasable
Achieve superior purity with the SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit. Utilizing a highly specific Column-based negative selection strategy, this kit effectively depletes non-target cells from Human PBMC using optimized Magnetic Beads.
SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) is a Indirect CD8+ T Cell Isolation Kit (Column-based, Non-releasable)
📄 MSDS

Informations de commande

N° de catalogue Nom du produit Taille Prix (EUR)
GM-CD8-T-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) 10 Tests EUR 176.70
GM-CD8-T-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) 100 Tests EUR 933.72
GM-CD8-T-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) 750 Tests
SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based)

Spécifications

  • Population cellulaire cible CD8-T Cell
  • Type de marquage Indirect
  • Type de bille Non-releasable
  • Format Column-based
  • Méthode d'isolement Negative CD8-T Cell Isolation (Untouched)
  • Conditions de stockage Conserver entre 2℃ et 8℃

Description

N° de catalogue GM-CD8-T-Cell-iso-kit
Nom du produit SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based)
Nom anglais SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based)
Description SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) is a Indirect CD8+ T Cell Isolation Kit (Column-based, Non-releasable)
Détails d'application SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) is used for Negative CD8+ T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Composants
  • GM-CD8-T-Cell-iso-Cocktail-1: SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Cocktail
  • GMP-SMT-219-Ab01-nanoIMB: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)
Type de marquage Indirect
Type de bille Non-releasable
Format Column-based
Caractéristique clé Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Population cellulaire cible CD8-T Cell
Conditions de stockage Conserver entre 2℃ et 8℃

Détails d'application

SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) is used for Negative CD8+ T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies

Protocole

  1. Preparation: Prepare a single-cell suspension from cryopreserved or fresh PBMCs.
  2. Labeling: Add the Biotin-Antibody Cocktail (targets non-CD8+ cells) to the cell suspension. Incubate.
  3. Bead Binding: Add Anti-Biotin Microbeads to bind the labeled non-target cells. Incubate.
  4. Separation: Apply the suspension to the separation column placed in a magnetic field.
  5. Elution: Unlabeled CD8+ T cells pass through the column freely and are collected in the flow-through fraction (Untouched).

Données de validation

Enrichissement efficace de cellules T CD8+ de haute pureté à partir de PBMC humains en utilisant SOLIDEX®-ISOEx Untouched Kit d'isolement de cellules T CD8+ humaines (basé sur colonne)

N° de catalogue GM-CD8-T-Cell-iso-kit
Pour évaluer l'efficacité d'isolement du SOLIDEX®-ISOEx Untouched Kit d'isolement de cellules T CD8+ humaines (basé sur colonne), les cellules T CD8+ ont été enrichies à partir de cellules mononucléées du sang périphérique humain (hPBMC) par sélection négative. Les cellules non cibles ont été marquées avec un cocktail d'anticorps biotinylés et déplétées à l'aide de billes magnétiques dans une colonne de séparation.
Les fractions cellulaires ont été colorées avec CD3-APC et CD8-PE pour une analyse par cytométrie en flux. La fréquence des cellules T CD8+ a montré une augmentation substantielle de 22,6% dans l'échantillon avant isolement à 91,9% dans la fraction après isolement. Ces résultats confirment que le système basé sur colonne offre une déplétion supérieure des populations non cibles, produisant des cellules T CD8+ hautement purifiées et « non touchées ».

Figure 17. Isolement de cellules T CD8+ humaines à l'aide de SOLIDEX®-ISOEx Untouched Kit d'isolement de cellules T CD8+ humaines (basé sur colonne).Graphiques de cytométrie en flux représentatifs montrant la fréquence des cellules T CD8+ dans les hPBMC avant isolement (22,6%) et après enrichissement par sélection négative sur colonne (91,9%).

ISOEx kit d'isolement cellulaire & Nanobeads validation
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Contexte

Why Choose SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit?

  • 100% Untouched Cells: Target CD8+ T cells are never bound by antibodies or beads, preventing receptor blocking and accidental activation.
  • Superior Purity: Column-based separation acts as a physical filter, significantly reducing background contamination compared to column-free methods.
  • High Viability: Gentle isolation preserves the metabolic health of cytotoxic T cells for sensitive functional assays.
  • Fast & Simple: Streamlined protocol allows for isolation in under 45 minutes.

FAQ

What are the sample requirements?
We recommend using fresh or cryopreserved Human PBMCs. For best results, ensure cell viability is >90% before starting. Leukapheresis products are also compatible.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (Negative Selection) kit, the CD8 receptors on the surface of your target cells are free and not blocked by any magnetic beads or antibodies. You can proceed directly to staining.
How does the Column-based separation work?
The column contains a ferromagnetic matrix. When placed in a magnetic field, it amplifies the magnetic force, effectively retaining the magnetically labeled non-target cells. The unlabeled CD8+ T cells simply pass through.
Are the separation columns reusable?
No. The columns are designed for single-use to ensure maximum sterility and prevent cross-contamination between patient samples.
What is the advantage of Column-based over Column-free methods?
Column-based methods typically offer higher purity. The column matrix allows for thorough washing of the retained cells, ensuring that only the specific "Untouched" cells pass through, whereas column-free methods can sometimes suffer from non-specific entrapment or lower purity in complex samples.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.

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