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SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)

🏷 GM-NK-Cell-iso-kit
IndirectColumn-basedNon-releasable
Achieve superior research results with the SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit. Designed for high-efficiency Human NK Cell purification from PBMC, this kit utilizes a specialized Column-based method with Magnetic Beads to ensure exceptional recovery and purity (up to 94.3%)(1). Our Untouched (Negative) selection strategy leaves target NK cells completely antibody- and bead-free, preserving their native state for functional assays. Ideal for immunology and cytotoxicity studies requiring High Purity and zero activation.
SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
📄 MSDS 📘 User Manual

订购信息

产品货号 产品名称 规格 价格 (USD)
GM-NK-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 10 Tests $190
GM-NK-Cell-iso-kit-50T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 50 Tests $570
GM-NK-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 100 Tests $1004
GM-NK-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 750 Tests
SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)

产品规格

  • 目标细胞群 NK Cell
  • 标记类型 Indirect
  • 磁珠类型 Non-releasable
  • 分选方式 Column-based
  • 分离方法 Negative NK Cell Isolation (Untouched)
  • 储存条件 2℃ - 8℃ 保存

产品详情

产品货号 GM-NK-Cell-iso-kit
产品名称 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)
英文名称 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)
产品详情 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
应用详情 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
组分
  • GM-NK-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
  • GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
标记类型 Indirect
磁珠类型 Non-releasable
分选方式 Column-based
关键特性 Preserves native cell function; maintains surface marker integrity; ideal for functional studies
目标细胞群 NK Cell
储存条件 2℃ - 8℃ 保存

应用详情

SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies

实验方案

  1. Preparation: Resuspend PBMCs in separation buffer.
  2. Labeling: Add the Isolation Cocktail and incubate for 15 minutes at 2-8°C(3).
  3. Washing: Wash cells with buffer to remove unbound antibodies(4).
  4. Bead Incubation: Add Anti-Biotin Nanobeads and incubate for 15 minutes at 2-8°C(5).
  5. Separation: Pass the suspension through the LS Column; collect the flow-through (enriched NK cells)(6).

验证数据

High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)

Catalog No.GM-Tg-hg-NK-Cell-iso-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to NK cell isolation using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) and Company M’s NK Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and recovery rate. SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) provides superior performance in isolating NK cells from human PBMCs, delivering high purity and recovery rate for downstream applications.

Figure 6. High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.

ISOEx Cell Isolation Kit & Nanobeads validation
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背景介绍

Why Choose SOLIDEX®-ISOEx?

  • Untouched Purity: Delivers high purity (validated data shows increase from ~8% to >94%) while keeping NK cells free of labels.
  • Preserved Functionality: The negative selection strategy ensures zero activation, maintaining the cell's native killing capacity.
  • Column-Based Efficiency: The column amplifies the magnetic field, ensuring superior depletion of non-target cells compared to column-free methods.
  • Fast Workflow: Optimized protocol with short incubation times allowing rapid isolation.

常见问题

What are the sample requirements? Can I use frozen PBMCs?
The kit is optimized for human PBMCs. Both fresh and cryopreserved PBMCs can be used. However, for frozen samples, ensure high viability (>90%) and remove clumps/debris with a strainer before labeling to prevent column clogging.

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