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SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)

🏷 GM-NK-Cell-iso-kit
IndirectColumn-basedNon-releasable
Achieve superior research results with the SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit. Designed for high-efficiency Human NK Cell purification from PBMC, this kit utilizes a specialized Column-based method with Magnetic Beads to ensure exceptional recovery and purity (up to 94.3%). Our Untouched (Negative) selection strategy leaves target NK cells completely antibody- and bead-free, preserving their native state for functional assays. Ideal for immunology and cytotoxicity studies requiring High Purity and zero activation.
SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
📄 MSDS 📘 User Manual

订购信息

产品货号 产品名称 规格 价格 (EUR)
GM-NK-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 10 Tests EUR 176.70
GM-NK-Cell-iso-kit-50T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 50 Tests EUR 530.10
GM-NK-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 100 Tests EUR 933.72
GM-NK-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 750 Tests
SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)

产品规格

  • 目标细胞群 NK Cell
  • 标记类型 Indirect
  • 磁珠类型 Non-releasable
  • 有无柱式 Column-based
  • 分离方法 Negative NK Cell Isolation (Untouched)
  • 储存条件 2℃ - 8℃ 保存

产品详情

产品货号 GM-NK-Cell-iso-kit
产品名称 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)
英文名称 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)
产品详情 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
应用详情 SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
组分
  • GM-NK-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
  • GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
标记类型 Indirect
磁珠类型 Non-releasable
有无柱式 Column-based
关键特性 Preserves native cell function; maintains surface marker integrity; ideal for functional studies
目标细胞群 NK Cell
储存条件 2℃ - 8℃ 保存

应用详情

SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies

实验方案

  1. Preparation: Resuspend PBMCs in separation buffer.
  2. Labeling: Add the Isolation Cocktail and incubate for 15 minutes at 2-8°C.
  3. Washing: Wash cells with buffer to remove unbound antibodies.
  4. Bead Incubation: Add Anti-Biotin Nanobeads and incubate for 15 minutes at 2-8°C.
  5. Separation: Pass the suspension through the LS Column; collect the flow-through (enriched NK cells)

验证数据

Isolation efficace des cellules NK à l'aide du kit d'isolation de cellules NK humaines SOLIDEX®-ISOEx Untouched (sur colonne)

N° de catalogue GM-NK-Cell-iso-kit
Pour déterminer la pureté des cellules NK isolées, les cellules NK CD56+ ont été triées magnétiquement à partir de cellules mononucléées du sang périphérique humain (PBMCs). Les cellules avant et après séparation ont été marquées avec des anticorps CD3-APC et CD56-PE puis analysées par cytométrie en flux. Les résultats ont montré une augmentation remarquable de la pureté des cellules NK CD56+ de 8.19% avant isolement à 94.3% après isolement, confirmant un enrichissement très efficace des cellules NK.

Figure 5. Isolation efficace des cellules NK à l'aide du kit d'isolation de cellules NK humaines SOLIDEX®-ISOEx Untouched (sur colonne).Les cellules non NK ont été marquées avec des anticorps biotinylés. Après incubation avec des nanobilles magnétiques anti-biotine, ces cellules ont été éliminées par une colonne d'isolation cellulaire. Les cellules NK ont été récoltées en collectant la fraction de passage. Les cellules NK dans les PBMCs humains pré-isolés. Des cellules NK de haute pureté ont été enrichies.

ISOEx kit d'isolement cellulaire & Nanobeads validation
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Isolation de haute pureté des cellules NK à partir de PBMCs humains à l'aide du kit d'isolation de cellules NK humaines SOLIDEX®-ISOEx Untouched (sur colonne)

N° de catalogue GM-Tg-hg-NK-Cell-iso-kit
Des cellules mononucléées du sang périphérique humain (PBMCs) ont été fraîchement préparées et soumises à une isolation des cellules NK à l'aide du kit d'isolation de cellules NK humaines SOLIDEX®-ISOEx Untouched (sur colonne) et du kit de cellules NK de la société M. La population cellulaire isolée a été analysée par cytométrie en flux pour évaluer la pureté et le taux de récupération. Le kit d'isolation de cellules NK humaines SOLIDEX®-ISOEx Untouched (sur colonne) offre des performances supérieures pour l'isolation des cellules NK à partir de PBMCs humains, fournissant une pureté et un taux de récupération élevés pour les applications en aval.

Figure 6. Isolation de haute pureté des cellules NK à partir de PBMCs humains à l'aide du kit d'isolation de cellules NK humaines SOLIDEX®-ISOEx Untouched (sur colonne).L'analyse par cytométrie en flux des cellules isolées à partir de PBMCs cryoconservés.

ISOEx kit d'isolement cellulaire & Nanobeads validation
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背景介绍

Why Choose SOLIDEX®-ISOEx?

  • Untouched Purity: Delivers high purity (validated data shows increase from ~8% to >94%) while keeping NK cells free of labels.
  • Preserved Functionality: The negative selection strategy ensures zero activation, maintaining the cell's native killing capacity.
  • Column-Based Efficiency: The column amplifies the magnetic field, ensuring superior depletion of non-target cells compared to column-free methods.
  • Fast Workflow: Optimized protocol with short incubation times allowing rapid isolation.

常见问题

Q1: What are the sample requirements? Can I use frozen PBMCs?
The kit is optimized for human PBMCs. Both fresh and cryopreserved PBMCs can be used. However, for frozen samples, ensure high viability (>90%) and remove clumps/debris with a strainer before labeling to prevent column clogging.
Q2: Since this is an Untouched selection, can I stain the cells immediately after isolation?
Yes. Because the NK cells in the flow-through are "untouched" (no antibodies or beads bound to them), all surface receptors are available. You can immediately proceed with flow cytometry staining or functional activation.
Q3: How does the Column-based separation work?
The column contains a matrix that amplifies the magnetic field. Magnetically labeled non-target cells are retained inside the column, while the unlabeled (untouched) NK cells pass through freely.
Q4: Can the columns be reused?
No. Columns are designed for single-use only to ensure sterility and prevent cross-contamination between samples.
Q5: What is the advantage of this Column-based method over Column-free methods?
Column-based isolation typically achieves higher purity because the column matrix physically traps weakly magnetic contaminants that might escape in a simple tube-based magnet system. It also allows for thorough washing to remove background noise.
Q6: Is this protocol compatible with automation?
Yes, the magnetic separation steps can be adapted to standard automated magnetic liquid handling workstations.

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