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SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based)

🏷 GM-Pan-T-Cell-iso-kit
IndirectColumn-basedNon-releasable
Achieve superior purity with GeneMedi’s SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit. Optimized for Human Pan-T Cell isolation from PBMC, this Column-based system utilizes a negative selection strategy to deliver truly "untouched" cells. Our biotin-conjugated antibody cocktail and Magnetic Beads remove non-T cells, ensuring the target CD3+ cells remain free of antibodies and bead residues. With High Purity (>95%) and zero activation, these cells are ideal for downstream functional assays.
SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) is a Indirect CD3+ Pan-T Cell Isolation Kit (Column-based, Non-releasable)
📄 MSDS

Informations de commande

N° de catalogue Nom du produit Taille Prix (EUR)
GM-Pan-T-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) 10 Tests EUR 176.70
GM-Pan-T-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) 100 Tests EUR 933.72
GM-Pan-T-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) 750 Tests
SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based)

Spécifications

  • Population cellulaire cible CD3+ Pan-T Cell
  • Type de marquage Indirect
  • Type de bille Non-releasable
  • Format Column-based
  • Méthode d'isolement Negative CD3+ Pan-T Cell Isolation (Untouched)
  • Conditions de stockage Conserver entre 2℃ et 8℃

Description

N° de catalogue GM-Pan-T-Cell-iso-kit
Nom du produit SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based)
Nom anglais SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based)
Description SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) is a Indirect CD3+ Pan-T Cell Isolation Kit (Column-based, Non-releasable)
Détails d'application SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) is used for Negative CD3+ Pan-T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Composants
  • GM-Pan-T-Cell-iso-Cocktail-1: SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Cocktail
  • GMP-SMT-219-Ab01-nanoIMB: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)
Type de marquage Indirect
Type de bille Non-releasable
Format Column-based
Caractéristique clé Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Population cellulaire cible CD3+ Pan-T Cell
Conditions de stockage Conserver entre 2℃ et 8℃

Détails d'application

SOLIDEX®-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) is used for Negative CD3+ Pan-T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies

Protocole

  1. Preparation: Isolate and count PBMCs; resuspend in separation buffer.
  2. Labeling: Add the Isolation Cocktail to cells and incubate for 5 minutes at room temperature.
  3. Magnetic Binding: Add Anti-biotin Nanobeads and incubate for 10 minutes at 2-8°C.
  4. Separation: Apply cell suspension to an LS Column on a magnetic separator. Collect the flow-through (this contains your enriched Pan-T cells).

Données de validation

Isolement de haute pureté des cellules Pan-T à partir de PBMC humains en utilisant le kit d'isolement de cellules Pan-T humaines SOLIDEX®-ISOEx Untouched (basé sur colonne)

N° de catalogue GM-Pan-T-Cell-iso-kit
Dans cette expérience, le kit d'isolement de cellules Pan-T humaines SOLIDEX®-ISOEx Untouched (basé sur colonne) a été utilisé pour isoler et purifier les cellules Pan-T à partir de cellules mononucléées du sang périphérique humain (PBMC). Tout d'abord, les cellules non Pan-T ont été spécifiquement marquées avec un cocktail d'anticorps conjugués à la biotine (Antibody Cocktail), suivi d'une incubation avec des billes nano-magnétiques anti-biotine. Lorsque la suspension cellulaire a traversé la colonne de séparation, les cellules non cibles marquées magnétiquement ont été retenues dans la colonne, tandis que les cellules Pan-T non marquées et intactes sont sorties avec le tampon d'élution. Les données ont montré que la proportion de cellules Pan-T a augmenté de manière significative, passant de 58,9% avant l'isolement à 96,1% après l'isolement. Les résultats expérimentaux démontrent que ce kit élimine efficacement divers types de cellules non Pan-T, produisant des cellules Pan-T de haute pureté qui conservent leur activité biologique d'origine.

Figure 12. Isolement de haute pureté des cellules Pan-T à partir de PBMC humains en utilisant le kit d'isolement de cellules Pan-T humaines SOLIDEX®-ISOEx Untouched (basé sur colonne).L'analyse par cytométrie en flux a été réalisée sur les populations cellulaires avant et après l'isolement. Les résultats confirment que cette méthode produit avec succès des cellules Pan-T de haute pureté.

ISOEx kit d'isolement cellulaire & Nanobeads validation
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Contexte

Why Choose SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit?

  • Truly Untouched: Uses negative selection to leave target T cells completely free of antibodies and magnetic particles, preserving their native function.
  • High Purity: Validated to achieve >98% purity (compared to ~58% in PBMCs).
  • Fast Workflow: Optimized rapid protocol with only 15 minutes of total incubation time.
  • High Compatibility: Compatible with standard LS columns and magnetic separators.

FAQ

What are the sample requirements?
This kit is optimized for Peripheral Blood Mononuclear Cells (PBMC). Best results are obtained with fresh PBMCs, but carefully cryopreserved PBMCs can also be used.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (negative) selection kit, the CD3+ T cells have no antibodies or beads attached to their surface. All surface epitopes are available for downstream antibody staining.
How does the column-based negative selection work?
Non-T cells (B cells, NK cells, Monocytes, etc.) are magnetically labeled and retained inside the column by the magnetic field. The Pan-T cells pass through the column freely and are collected in the flow-through.
Can the columns be reused?
No. The columns are designed for single-use to ensure sterility and prevent cross-contamination between samples.
What is the advantage of this column-based method over column-free methods?
Column-based separation typically offers higher purity due to the stronger magnetic gradient generated within the column matrix, which more effectively retains the magnetically labeled non-target cells compared to simple tube magnets.
Is this workflow compatible with automation?
Yes, the magnetic separation steps can be adapted to automated liquid handling systems that support magnetic bead separation or automated column separators.

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