SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free)
订购信息
| 产品货号 | 产品名称 | 规格 | 价格 (USD) |
|---|---|---|---|
| GM-Pan-T-Cell-iso-kit-CF-10T | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) | 10 Tests | $190 |
| GM-Pan-T-Cell-iso-kit-CF-50T | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) | 50 Tests | $570 |
| GM-Pan-T-Cell-iso-kit-CF-100T | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) | 100 Tests | $1004 |
| GM-Pan-T-Cell-iso-kit-CF-750T | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) | 750 Tests |
产品规格
- 目标细胞群 Pan-T Cell
- 标记类型 Indirect
- 磁珠类型 Non-releasable
- 分选方式 Column-free
- 分离方法 Negative Pan-T Cell Isolation (Untouched)
- 储存条件 2℃ - 8℃ 保存
产品详情
| 产品货号 | GM-Pan-T-Cell-iso-kit-CF |
| 产品名称 | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) |
| 英文名称 | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) |
| 产品详情 | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) is a Indirect Pan-T Cell Isolation Kit (Column-free, Non-releasable) |
| 应用详情 | SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) is used for Negative Pan-T Cell Isolation (Untouched), with the Key Features, such as Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications |
| 组分 |
|
| 标记类型 | Indirect |
| 磁珠类型 | Non-releasable |
| 分选方式 | Column-free |
| 关键特性 | Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications |
| 目标细胞群 | Pan-T Cell |
| 储存条件 | 2℃ - 8℃ 保存 |
验证数据
Superior Isolation of High-Purity CD3+ T Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free)
Catalog No. GM-Pan-T-Cell-iso-kit-CF
To evaluate the isolation efficiency of the SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free), CD3+ T cells were enriched from human peripheral blood mononuclear cells (hPBMCs) via negative selection. In this column-free protocol, non-target cells were labeled with a biotinylated antibody cocktail followed by magnetic beads. The labeled cells were then sequestered by a magnetic separator, allowing the highly purified, untouched CD3+ T cells to be recovered directly from the supernatant. Cell fractions were stained with CD56-PE and CD3-APC for flow cytometric analysis. The frequency of CD3+ T cells demonstrated a substantial increase from 40.7% in the pre-isolation sample to 92.5% in the post-isolation fraction. These results confirm that the column-free system provides effective depletion of non-target populations, yielding high-purity CD3+ T cells while maintaining their native state and maximal viability.
Figure 21. Rapid isolation and high-purity enrichment of Human CD3+ T Cells Using SOLIDEX®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free).Representative flow cytometry plots showing the frequency of CD3+ T cells in hPBMCs before isolation (40.7%) and after enrichment via column-free negative selection (92.5%).
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