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SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based)

🏷 GM-Tg-hg-MP2610-dep-kit
IndirectColumn-basedNon-releasable
Achieve superior enrichment of untouched TCR γδT cells, NK cells, or B cells by efficiently removing Human TCR alpha-beta (abT)+ Cells from PBMC. This Depletion kit utilizes a high-performance Column-based strategy combined with indirect Magnetic Beads labeling. By effectively trapping the unwanted αβ T cells in the column, the flow-through fraction contains the target cells of interest. The result is High Purity non-TCR αβ cells with no antibody/bead residue and Zero Activation, perfectly preserving their physiological state.
SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) is a Indirect TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based, Non-releasable)
📄 MSDS

Informations de commande

N° de catalogue Nom du produit Taille Prix (EUR)
GM-Tg-hg-MP2610-dep-kit-10T SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) 10 Tests EUR 104.16
GM-Tg-hg-MP2610-dep-kit-100T SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) 100 Tests EUR 549.63
GM-Tg-hg-MP2610-dep-kit-750T SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) 750 Tests

Spécifications

  • Population cellulaire cible TCR+ Cell
  • Type de marquage Indirect
  • Type de bille Non-releasable
  • Format Column-based
  • Méthode d'isolement TCR alpha-beta (abT)+ Cell Depletion
  • Conditions de stockage Conserver entre 2℃ et 8℃

Description

N° de catalogue GM-Tg-hg-MP2610-dep-kit
Nom du produit SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based)
Nom anglais SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based)
Description SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) is a Indirect TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based, Non-releasable)
Détails d'application SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) is used for TCR alpha-beta (abT)+ Cell Depletion, with the Key Features, such as Specific cell depletion; minimal non-specific binding; optimal for complex samples
Composants
  • GM-Tg-hg-MP2610-Ab-3-Biotin : SOLIDEX®: SOLIDEX®-ISOEx Anti- Human TCR alpha-beta (abT)+ Cell monoclonal antibody(mAb) (Biotin)
  • GMP-SMT-219-Ab01-nanoIMB: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)
Type de marquage Indirect
Type de bille Non-releasable
Format Column-based
Caractéristique clé Specific cell depletion; minimal non-specific binding; optimal for complex samples
Population cellulaire cible TCR+ Cell
Conditions de stockage Conserver entre 2℃ et 8℃

Détails d'application

SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) is used for TCR alpha-beta (abT)+ Cell Depletion, with the Key Features, such as Specific cell depletion; minimal non-specific binding; optimal for complex samples

Protocole

  1. Preparation: Prepare a single-cell suspension from Human PBMCs.
  2. Labeling: Incubate cells with the primary antibody cocktail targeting TCR αβ.
  3. Bead Binding: Add the Anti-Biotin/Secondary Nanobeads to bind the primary antibodies.
  4. Separation: Apply the suspension to a column placed in a magnetic field.
  5. Collection (Depletion): The unwanted TCR αβ+ cells are retained in the column. Collect the flow-through, which contains the untouched target cells (e.g., γδT cells, NK cells).

Données de validation

Déplétion efficace des cellules T TCR alpha-bêta (αβ)+ à partir de PBMC humains à l'aide du kit de déplétion indirecte de cellules TCR alpha-bêta (αβ)+ humaines SOLIDEX®-ISOEx (basé sur colonne)

Numéro de catalogue GM-Tg-hg-MP2610-dep-kit
Cette expérience a été conçue pour évaluer l'efficacité de déplétion du kit de déplétion indirecte de cellules TCR alpha-bêta (αβ)+ humaines SOLIDEX®-ISOEx (basé sur colonne) pour éliminer les cellules TCR alpha-bêta (αβ)+. L'analyse des données a démontré l'excellente performance de déplétion du kit. Dans l'échantillon initial de PBMC, les cellules TCR alpha-bêta (αβ)+ représentaient 54,7%. Après passage à travers la colonne de séparation, les cellules TCR alpha-bêta (αβ)+ résiduelles dans la fraction de passage ont considérablement diminué à 0,079%, indiquant une efficacité de déplétion supérieure à 99,3%. Ces résultats confirment que le kit réalise une déplétion hautement efficace avec une excellente spécificité, sans provoquer de perte non spécifique des cellules non cibles.

Figure 11. Déplétion efficace des cellules TCR alpha-bêta (αβ)+ à partir de PBMC humains à l'aide du kit de déplétion indirecte de cellules TCR alpha-bêta (αβ)+ humaines SOLIDEX®-ISOEx (basé sur colonne).Les résultats montrent que la proportion de cellules TCR alpha-bêta (αβ)+ était de 54,7% avant la déplétion (Pré-déplétion) et a diminué à 0,079% après la déplétion (Après déplétion / Passage). Cela démontre que jusqu'à 99,3% des cellules TCR alpha-bêta (αβ)+ ont été capturées et retenues par la colonne de séparation magnétique, confirmant la haute spécificité de la capture par billes pour les cellules TCR alpha-bêta (αβ)+.

ISOEx kit d'isolement cellulaire & Nanobeads validation
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Contexte

Why Choose SOLIDEX®-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit?

  • Pristine "Untouched" Cells: The target cells in the flow-through are never bound by antibodies or beads, ensuring zero activation and native phenotype.
  • High Depletion Efficiency: The column-based high-gradient magnetic field ensures rigorous trapping of TCR αβ cells, typically removing >99% of contaminants.
  • Flexible Strategy: The indirect labeling method allows for high-sensitivity recognition of the TCR αβ complex.
  • Downstream Compatibility: Perfect for immediate flow cytometry, functional culture, or molecular analysis.

FAQ

What are the sample requirements?
This kit is optimized for fresh or cryopreserved Human PBMCs. It can also be used with Leukapheresis products. For frozen samples, ensure proper DNase treatment to prevent clumping.
Can I stain the isolated cells for Flow Cytometry immediately?
Yes. Since this is a depletion strategy, the cells in the flow-through (your target cells) have no magnetic beads or antibodies attached. Their surface markers are free and available for staining.
How does the Column-based separation work for depletion?
The column contains a ferromagnetic matrix. When placed in a magnetic field, it creates a high-gradient trap. This securely holds the magnetically labeled unwanted cells (TCR $αβ+$), preventing them from leaking into the flow-through, which ensures high purity of the non-magnetic fraction.
Are the separation columns reusable?
No. The columns are single-use to ensure sterility and prevent cross-contamination between samples.
What is the advantage of Column-based depletion over Column-free methods?
Column-based depletion generally offers higher depletion efficiency. The physical matrix traps unwanted cells more securely than simple tube-based magnetic attraction, reducing the risk of contaminating your final "untouched" sample with residual TCR αβ cells.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with standard automated magnetic separation instruments designed for column-based protocols.

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