SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based)
Unlock high-efficiency isolation of Human CD4+ T Cells (Helper T Cells) with SOLIDEX®-ISOEx Nanobeads. Utilizing a robust Positive Selection strategy, this kit specifically targets CD4 antigens to recover high-yield cell populations directly from PBMC. The Column-based format combined with biodegradable Magnetic Beads allows for rigorous washing, ensuring High Purity and minimal background. The gentle isolation process preserves cell viability, making it ideal for HIV research, cytokine analysis, and immune profiling.
SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is a Direct CD4+ T Cell Isolation Nanobeads (Column-based, Non-releasable)
Informations de commande
| N° de catalogue | Nom du produit | Taille | Prix (EUR) |
|---|---|---|---|
| GM-Tg-hg-T10191-iso-nanoIMB-10T | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) | 10 Tests | EUR 104.16 |
| GM-Tg-hg-T10191-iso-nanoIMB-100T | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) | 100 Tests | EUR 549.63 |
| GM-Tg-hg-T10191-iso-nanoIMB-750T | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) | 750 Tests |
Spécifications
- Population cellulaire cible CD4-T Cell
- Type de marquage Direct
- Type de bille Non-releasable
- Format Column-based
- Méthode d'isolement Positive CD4-T Cell Selection
- Conditions de stockage Conserver entre 2℃ et 8℃
Description
| N° de catalogue | GM-Tg-hg-T10191-iso-nanoIMB |
| Nom du produit | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) |
| Nom anglais | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) |
| Description | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is a Direct CD4+ T Cell Isolation Nanobeads (Column-based, Non-releasable) |
| Détails d'application | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is used for Positive CD4+ T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows |
| Type de marquage | Direct |
| Type de bille | Non-releasable |
| Format | Column-based |
| Caractéristique clé | Fast isolation; high purity; ideal for automated workflows |
| Population cellulaire cible | CD4-T Cell |
| Conditions de stockage | Conserver entre 2℃ et 8℃ |
Détails d'application
SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is used for Positive CD4+ T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows
Protocole
- Preparation: Prepare a single-cell suspension from Human PBMCs (fresh or frozen).
- Labeling: Add CD4 Microbeads directly to the cell suspension. Incubate to bind CD4+ T Cells.
- Separation: Place a separation column in the magnetic field and apply the cell suspension.
- Washing: Magnetically labeled CD4+ cells are retained in the column; unlabeled cells (CD8 T cells, B cells, etc.) are washed away.
- Elution: Remove the column from the magnet and elute the purified CD4+ T Cells.
Données de validation
Isolation efficace des cellules T CD4+ à l'aide des nanobilles d'isolation SOLIDEX®-ISOEx Human CD4 (basée sur colonne)
N° de catalogue GM-Tg-hg-T10191-iso-nanoIMB
Pour évaluer la pureté des cellules T CD4+ isolées, une séparation magnétique a été réalisée sur des cellules mononucléées du sang périphérique humain (PBMCs). Les cellules avant et après isolation ont été marquées avec des anticorps CD3-APC et CD4-PE et soumises à une analyse par cytométrie en flux. La pureté des cellules T CD4+ a été considérablement améliorée, passant de 28,1 % avant isolation à 97,0 % après isolation, indiquant un enrichissement très efficace des cellules T CD4+.
Figure 9. Enrichissement à haute efficacité des cellules CD4+ avec les nanobilles d'isolation SOLIDEX®-ISOEx Human CD4 (basée sur colonne).Les cellules ont été incubées avec des nanobilles magnétiques conjuguées à l'anticorps anti-CD4 et passées à travers une colonne d'isolation pour une sélection positive. La cytométrie en flux montre les cellules T CD4+ dans la population de PBMC d'entrée et la fraction hautement purifiée résultante.
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Contexte
Why Choose SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads?
- Maximum Recovery: Positive selection efficiently captures target cells even from samples with low frequency.
- High Specificity: Column technology enables thorough washing, significantly reducing non-specific binding compared to clump-based methods.
- Bio-friendly Nanobeads: Biodegradable beads minimize receptor cross-linking, preventing artificial activation of sensitive Helper T cells.
- Standardized Workflow: Reliable results in under 45 minutes, compatible with downstream cell culture.
FAQ
What are the sample requirements?
The kit is optimized for fresh or cryopreserved Human PBMCs. It is also highly effective with Leukapheresis products (Leukopaks). Ensure cells are well-dispersed and viable (>90%) for best results.
Do the magnetic beads affect cell activation?
No. The SOLIDEX® nanobeads are biodegradable and nano-sized. They are engineered to bind without inducing significant signal transduction or activation, ensuring the CD4+ cells remain phenotypically unaltered for functional assays.
How does the Column-based separation work?
The column contains a matrix of ferromagnetic spheres. When placed in a magnetic field, these spheres amplify the magnetic gradient, trapping the bead-labeled CD4+ cells. This strong retention allows for vigorous washing of contaminants before the target cells are released.
Are the separation columns reusable?
No. To maintain sterility and absolutely prevent cross-contamination between donor samples, the columns are designed for single-use only.
What is the advantage of Column-based over Column-free methods?
Column-based positive selection generally delivers higher purity. The physical column matrix allows for the washing away of non-specific cells that might get trapped in the "clumps" formed by column-free tube methods.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is designed to be compatible with most standard automated magnetic cell separators using column technology.
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