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SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)

🏷 GM-Tg-hg-T23212-iso-kit-1
IndirectColumn-basedNon-releasable
Isolate pristine Human Classical Monocytes (CD14+CD16-) with the SOLIDEX®-ISOEx Kit. Utilizing a superior Column-based Negative (Untouched) selection strategy, this kit depletes non-target cells from PBMC using optimized Magnetic Beads. The eluted cells are free of antibodies and beads, ensuring Zero Activation and preserving their native phenotype. Achieve High Purity and high viability for sensitive downstream applications like macrophage differentiation.
SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable)
📄 MSDS

Informations de commande

N° de catalogue Nom du produit Taille Prix (EUR)
GM-Tg-hg-T23212-iso-kit-1-10T SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 10 Tests EUR 176.70
GM-Tg-hg-T23212-iso-kit-1-100T SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 100 Tests EUR 933.72
GM-Tg-hg-T23212-iso-kit-1-750T SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 750 Tests
SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)

Spécifications

  • Population cellulaire cible CD14+CD16- Cell
  • Type de marquage Indirect
  • Type de bille Non-releasable
  • Format Column-based
  • Méthode d'isolement Negative Classical Monocyte Isolation (Untouched)
  • Conditions de stockage Conserver entre 2℃ et 8℃

Description

N° de catalogue GM-Tg-hg-T23212-iso-kit-1
Nom du produit SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)
Nom anglais SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)
Description SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable)
Détails d'application SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is used for Negative CD14+ Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Composants
  • GM-Tg-hg-T23212-iso-Cocktail-1: SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Cocktail
  • GMP-SMT-219-Ab01-nanoIMB: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)
Type de marquage Indirect
Type de bille Non-releasable
Format Column-based
Caractéristique clé Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Population cellulaire cible CD14+CD16- Cell
Conditions de stockage Conserver entre 2℃ et 8℃

Détails d'application

SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is used for Negative CD14+ Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies

Protocole

  1. Preparation: Prepare a single-cell suspension from cryopreserved or fresh PBMCs.
  2. Labeling: Add the Biotin-Antibody Cocktail (targets non-CD14 cells: T cells, B cells, NK cells, etc.). Incubate.
  3. Bead Binding: Add Anti-Biotin Microbeads to bind the labeled non-target cells. Incubate.
  4. Separation: Apply the suspension to the separation column placed in a magnetic field.
  5. Elution: Unlabeled CD14+ Classical Monocytes pass through the column freely and are collected (Untouched).

Données de validation

Isolement de haute pureté des monocytes classiques à partir de cellules mononucléées du sang périphérique humain (PBMC) en utilisant le SOLIDEX®-ISOEx Untouched Kit d'isolement des monocytes classiques humains (sur colonne)

N° de catalogue GM-Tg-hg-T23212-iso-kit-1
Dans cette expérience, le SOLIDEX®-ISOEx Untouched Kit d'isolement des monocytes classiques humains (sur colonne) a été utilisé pour isoler et purifier les monocytes classiques à partir de PBMC cryoconservés. Premièrement, les cellules non monocytaires ont été spécifiquement marquées avec un cocktail d'anticorps conjugués à la biotine (Antibody Cocktail), suivi d'une incubation avec des billes nano-magnétiques anti-biotine. La pureté des populations cellulaires avant et après isolement a été analysée par cytométrie en flux. La pureté des monocytes classiques CD14+ dans la fraction non retenue est passée de 21,7 % à 92,1 %. Les résultats expérimentaux montrent que ce kit élimine efficacement les cellules contaminantes, produisant des monocytes classiques de haute pureté, non touchés (non liés aux anticorps).

Figure 14. Isolement de haute pureté des monocytes classiques à partir de cellules mononucléées du sang périphérique humain (PBMC) en utilisant le SOLIDEX®-ISOEx Untouched Kit d'isolement des monocytes classiques humains (sur colonne).Les résultats de cytométrie en flux montrent que la pureté des monocytes classiques CD14+ est passée de 21,7 % à 92,1 %.

ISOEx kit d'isolement cellulaire & Nanobeads validation
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Contexte

Why Choose SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit?

  • True "Untouched" Isolation: Target monocytes are never bound by antibodies or beads, preventing Fc-receptor activation and preserving differentiation potential.
  • Exceptional Purity: The Column-based matrix acts as a physical filter, allowing rigorous washing of contaminants to achieve >90% purity compared to column-free methods.
  • Preserved Physiology: Gentle isolation ensures cells remain in a resting state, critical for accurate immunological studies.
  • Rapid Workflow: Isolate cells in under 45 minutes with a simple, standardized protocol.

FAQ

What are the sample requirements?
We recommend using fresh or cryopreserved Human PBMCs. For cryopreserved samples, ensure high viability (>90%) and proper DNase treatment during thawing to prevent clumping. Leukapheresis products are also compatible.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (Negative Selection) kit, the CD14 receptors and other surface markers are free and not blocked by magnetic beads or antibodies. You can proceed directly to staining.
How does the Column-based separation work?
The column contains a ferromagnetic matrix (spheres). When placed in a magnetic field, the matrix amplifies the magnetic force by 10,000-fold, effectively retaining the magnetically labeled non-target cells while unlabeled monocytes pass through.
Are the separation columns reusable?
No. The columns are designed for single-use to ensure sterility and prevent cross-contamination between patient samples.
What is the advantage of Column-based over Column-free methods?
Column-based methods typically offer higher purity. The column allows for washing of the retained cell mass, ensuring that only the specific "Untouched" cells elute. Column-free methods often struggle with non-specific entrapment of target cells in the magnetic clump, leading to lower purity or recovery.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.

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