SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based)
Maximize your cell recovery with SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads. Employing a highly efficient Positive Selection strategy, this kit is designed to isolate Human CD3+ T Cells directly from PBMC with exceptional yield. The Column-based technology paired with specific Magnetic Beads allows for rigorous washing, resulting in High Purity cell populations. The biodegradable nanobeads ensure minimal interference, making the isolated cells ideal for molecular analysis and cell expansion.
SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is a Direct Pan-T Cell Isolation Nanobeads (Column-based, Non-releasable)
Informations de commande
| N° de catalogue | Nom du produit | Taille | Prix (EUR) |
|---|---|---|---|
| GM-Tg-hg-T87075-iso-nanoIMB-10T | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) | 10 Tests | EUR 104.16 |
| GM-Tg-hg-T87075-iso-nanoIMB-100T | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) | 100 Tests | EUR 549.63 |
| GM-Tg-hg-T87075-iso-nanoIMB-750T | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) | 750 Tests |
Spécifications
- Population cellulaire cible Pan-T Cell
- Type de marquage Direct
- Type de bille Non-releasable
- Format Column-based
- Méthode d'isolement Positive Pan-T Cell Selection
- Conditions de stockage Conserver entre 2℃ et 8℃
Description
| N° de catalogue | GM-Tg-hg-T87075-iso-nanoIMB |
| Nom du produit | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) |
| Nom anglais | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) |
| Description | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is a Direct Pan-T Cell Isolation Nanobeads (Column-based, Non-releasable) |
| Détails d'application | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is used for Positive Pan-T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows |
| Type de marquage | Direct |
| Type de bille | Non-releasable |
| Format | Column-based |
| Caractéristique clé | Fast isolation; high purity; ideal for automated workflows |
| Population cellulaire cible | Pan-T Cell |
| Conditions de stockage | Conserver entre 2℃ et 8℃ |
Détails d'application
SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is used for Positive Pan-T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows
Protocole
- Preparation: Prepare a single-cell suspension from Human PBMCs.
- Labeling: Add CD3 Microbeads directly to the cell suspension. Incubate to allow binding to CD3+ T Cells.
- Separation: Apply the suspension to a separation column placed in a magnetic field.
- Washing: The labeled CD3+ cells are retained in the column, while unlabeled cells (B cells, NK cells, Monocytes) are washed away.
- Elution: Remove the column from the magnetic field and elute the highly purified CD3+ T Cells.
Données de validation
Isolement efficace des cellules Pan-T à l'aide de nanobilles d'isolement humaines SOLIDEX®-ISOEx CD3 (basé sur colonne)
N° de catalogue GM-Tg-hg-T87075-iso-nanoIMB
Pour évaluer la pureté des cellules T isolées, les cellules T CD3+ ont été triées magnétiquement à partir de cellules mononucléées du sang périphérique humain (PBMC). Les cellules avant et après séparation ont été colorées avec l'anticorps APC-conjugated CD3 et de l'iodure de propidium (PI), suivi d'une analyse par cytométrie en flux. Les résultats ont démontré une augmentation significative de la pureté des cellules T CD3+ de 52.6% avant isolement à 95.0% après isolement, confirmant un isolement réussi des cellules T.
Figure 7. Isolement efficace des cellules Pan-T à l'aide de nanobilles d'isolement humaines SOLIDEX®-ISOEx CD3 (basé sur colonne).Les cellules T ont été marquées avec des nanobilles SOLIDEX®-ISOEx conjuguées à un anticorps anti-CD3. Ensuite, ces cellules ont été enrichies en utilisant une colonne d'isolement cellulaire. Les cellules T dans les PBMC humains avant isolement. Des cellules T avec une pureté et une motilité élevées ont été enrichies.
Cliquez pour agrandir
Contexte
Why Choose SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads?
- Superior Recovery: Positive selection strategy ensures the capture of the maximum number of target CD3+ T cells, ideal for rare samples.
- High Specificity: The column-based washing step efficiently removes background cells, delivering exceptional purity.
- Biodegradable Nanobeads: The tiny magnetic beads are non-toxic and biodegradable, minimizing interference with cellular physiology.
- Fast Protocol: Simple incubation and separation steps allow for isolation in approximately 45 minutes.
FAQ
What are the sample requirements?
This kit is optimized for use with fresh or cryopreserved Human PBMCs. It is also compatible with Leukapheresis products (Leukopaks). Ensure high cell viability for optimal recovery.
Do the magnetic beads affect cell activation?
No. The SOLIDEX® nanobeads are extremely small and biodegradable. They are designed to minimize cross-linking and avoid inducing artificial activation of the T cells, making them safe for downstream culture and analysis.
How does the Column-based separation work?
The column contains a ferromagnetic matrix. When placed in a magnetic field, it amplifies the magnetic force, trapping the magnetically labeled CD3+ cells. This allows for vigorous washing to remove unlabeled contaminants before the target cells are eluted.
Are the separation columns reusable?
No. The columns are strictly single-use to ensure sterility and prevent cross-contamination between patient samples or experimental conditions.
What is the advantage of Column-based over Column-free methods?
Column-based positive selection typically offers higher purity. The physical matrix allows for thorough washing of the retained cells, removing non-specifically trapped contaminants that often remain in column-free "clump" based methods.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.
Besoin de plus de données ou d'études de cas sur ce produit ?
✉ Contactez-nous / Obtenir un devis