SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based)
Achieve superior isolation of Human CD8+ T Cells (Cytotoxic T Lymphocytes) with SOLIDEX®-ISOEx Nanobeads. Utilizing a high-efficiency Positive Selection strategy, this kit specifically targets CD8 antigens to maximize cell recovery from PBMC or Leukopaks. The Column-based technology combined with advanced Magnetic Beads allows for rigorous washing steps, ensuring High Purity and eliminating non-specific background. These biodegradable nanobeads preserve cell viability, making the isolated cells perfect for tumor killing assays and CAR-T development.
SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) is a Direct CD8+ T Cell Isolation Nanobeads (Column-based, Non-releasable)
Informations de commande
| N° de catalogue | Nom du produit | Taille | Prix (EUR) |
|---|---|---|---|
| GM-Tg-hg-TA040-iso-nanoIMB-10T | SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) | 10 Tests | EUR 104.16 |
| GM-Tg-hg-TA040-iso-nanoIMB-100T | SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) | 100 Tests | EUR 549.63 |
| GM-Tg-hg-TA040-iso-nanoIMB-750T | SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) | 750 Tests |
Spécifications
- Population cellulaire cible CD8-T Cell
- Type de marquage Direct
- Type de bille Non-releasable
- Format Column-based
- Méthode d'isolement Positive CD8-T Cell Selection
- Conditions de stockage Conserver entre 2℃ et 8℃
Description
| N° de catalogue | GM-Tg-hg-TA040-iso-nanoIMB |
| Nom du produit | SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) |
| Nom anglais | SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) |
| Description | SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) is a Direct CD8+ T Cell Isolation Nanobeads (Column-based, Non-releasable) |
| Détails d'application | SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) is used for Positive CD8+ T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows |
| Type de marquage | Direct |
| Type de bille | Non-releasable |
| Format | Column-based |
| Caractéristique clé | Fast isolation; high purity; ideal for automated workflows |
| Population cellulaire cible | CD8-T Cell |
| Conditions de stockage | Conserver entre 2℃ et 8℃ |
Détails d'application
SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads (Column-based) is used for Positive CD8+ T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows
Protocole
- Preparation: Prepare a single-cell suspension from Human PBMCs (fresh or frozen).
- Labeling: Add CD8 Microbeads directly to the cell suspension. Incubate to bind CD8+ T Cells.
- Separation: Place a separation column in the magnetic field and apply the cell suspension.
- Washing: The magnetically labeled CD8+ cells are retained in the column; unlabeled cells (CD4+ T cells, B cells, Monocytes) are washed away.
- Elution: Remove the column from the magnet and elute the highly purified CD8+ T Cells.
Données de validation
Isolation efficace des cellules T CD8+ à l'aide des nanobilles d'isolation SOLIDEX®-ISOEx Human CD8 (basées sur colonne)
N° de catalogue GM-Tg-hg-TA040-iso-nanoIMB
Pour déterminer la pureté des cellules T CD8+ isolées, les cellules T CD8+ ont été triées magnétiquement à partir de cellules mononucléées du sang périphérique humain (PBMC). Les cellules avant et après séparation ont été marquées avec des anticorps CD3-APC et CD8-PE puis analysées par cytométrie en flux. Les résultats ont montré une augmentation remarquable de la pureté des cellules T CD8+ de 16,6 % avant isolation à 94,6 % après isolation, confirmant un enrichissement très efficace des cellules T CD8+.
Figure 10. Isolation efficace des cellules CD8+ à l'aide des nanobilles d'isolation SOLIDEX®-ISOEx Human CD8 (basées sur colonne).Les cellules T ont été marquées avec des nanobilles magnétiques conjuguées à un anticorps anti-CD8. Ensuite, ces cellules ont été enrichies à l'aide d'une colonne d'isolation cellulaire. Les cellules T dans les PBMC humains pré-isolés. Des cellules T de haute pureté ont été enrichies.
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Contexte
Why Choose SOLIDEX®-ISOEx Human CD8 Isolation Nanobeads?
- Exceptional Recovery: Positive selection captures the maximum number of target CD8+ cells, essential when working with limited patient samples.
- Superior Specificity: The column matrix allows for thorough washing, significantly reducing contamination compared to column-free "clump" methods.
- Biodegradable Nanobeads: The tiny, non-toxic beads degrade naturally and do not over-stimulate the cells, preserving their cytotoxic potential.
- Rapid Workflow: Isolate cells in approximately 45 minutes with a simple, standardized protocol.
FAQ
What are the sample requirements?
This kit is optimized for fresh or cryopreserved Human PBMCs. It is also fully compatible with Leukapheresis products (Leukopaks). Ensure high cell viability (>90%) and proper DNase treatment if using frozen samples to prevent clumping.
Do the magnetic beads affect cell activation?
No. The SOLIDEX® nanobeads are biodegradable and extremely small (nano-scale). They are designed to minimize receptor cross-linking and avoid inducing artificial activation, ensuring your CD8+ cells remain in a physiological state for downstream functional assays.
How does the Column-based separation work?
The column contains a matrix of ferromagnetic spheres. When placed in a magnetic field, these spheres amplify the magnetic gradient, effectively trapping the bead-labeled CD8+ cells. This allows non-target cells to be washed away freely.
Are the separation columns reusable?
No. The columns are strictly single-use to ensure sterility and prevent cross-contamination between patient samples or experimental conditions.
What is the advantage of Column-based over Column-free methods?
Column-based positive selection typically yields higher purity. The physical column allows for active washing of the retained cells, removing contaminants that are often trapped non-specifically in the aggregates formed by column-free methods.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic cell separation instruments that utilize column technology.
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